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Reference: Gey U, et al. (2008) Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases. J Biol Chem 283(15):9759-67

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Abstract


The activity of yeast pyruvate dehydrogenase complex is regulated by reversible phosphorylation. Recently, we identified two enzymes which are involved in the phosphorylation (Pkp1p) and dephosphorylation (Ppp1p) of Pda1p, the alpha-subunit of the pyruvate dehydrogenase complex. We here provide evidence that two additional mitochondrial proteins, Pkp2p (Ygl059wp) and Ppp2p (Ycr079wp), are engaged in the regulation of this complex by affecting the phosphorylation state of Pda1p. Our data indicate complementary activities of the kinases and a redundant function for the phosphatases. Both proteins are associated with the complex. We propose a model for the role of the regulatory enzymes and the phosphorylation state of Pda1p in the assembly process of the pyruvate dehydrogenase complex.

Reference Type
Journal Article
Authors
Gey U, Czupalla C, Hoflack B, Rodel G, Krause-Buchholz U
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