Reference: Ashe M, et al. (2008) The SBF- and MBF-associated Protein Msa1 Is Required for Proper Timing of G1-specific Transcription in Saccharomyces cerevisiae. J Biol Chem 283(10):6040-9

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Abstract

In the budding yeast Saccharomyces cerevisiae, cell cycle initiation is prompted during G1 phase by Cln3/CDK1-mediated transcriptional activation of G1-specific genes. A recent screening performed to reveal novel interactors of SBF and MBF identified, in addition to the SBF-specific repressor Whi5 and the MBF-specific corepressor Nrm1, a pair of homologous proteins, Msa1 and Msa2 (encoded by YOR066w and YKR077w), as interactors of SBF and MBF, respectively. MSA1 is expressed periodically during the cell cycle with peak mRNA levels occurring at late M/early G1 and peak protein levels occurring in early G1. Msa1 associates with SBF and MBF-regulated target promoters consistent with a role in G1-specific transcriptional regulation. Msa1 affects cell cycle initiation by advancing the timing of transcription of G1-specific genes. Msa1 binds to SBF- and MBF-regulated promoters and binding is maximal during G1 phase. Binding depends upon the cognate transcription factor. Msa1 overexpression advances the timing of SBF-dependent transcription and budding whereas depletion delays both indicators of cell cycle initiation. Similar effects on MBF-regulated transcription are observed. Based upon these results, we conclude that Msa1 acts to advance the timing of G1-specific transcription and cell cycle initiation.

Reference Type
Journal Article
Authors
Ashe M, de Bruin RA, Kalashnikova TI, McDonald WH, Yates JR 3rd, Wittenberg C
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