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Reference: Fekete Z, et al. (2008) Pet127 Governs a 5' -> 3'-Exonuclease Important in Maturation of Apocytochrome b mRNA in Saccharomyces cerevisiae. J Biol Chem 283(7):3767-72

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Abstract

The details of mRNA maturation in Saccharomyces mitochondria are not well understood. All seven mRNAs are transcribed as part of multigenic units. The mRNAs are processed at a common 3' dodecamer sequence, but the 5' ends have seven different sequences. To investigate whether apocytochrome b (COB) mRNA is processed at the 5' end from a longer precursor by an endonuclease or an exonuclease, a 64-nucleotide sequence, which is required for the protection of COB mRNA by the Cbp1 protein and is found at the 5' end of the processed COB mRNA, was duplicated in tandem. The wild-type 64-nucleotide element functioned in either the upstream or downstream position when paired with a mutant element. In the tandem wild type strain, the 5' end of the mRNA was at the 5' end of the upstream unit, demonstrating that the mRNA is processed by an exonuclease. Accumulation of precursor COB RNA in single and double element strains with a deletion of PET127 demonstrated that the encoded protein governs the 5' exonuclease responsible for processing the precursor to the mature form.

Reference Type
Journal Article
Authors
Fekete Z, Ellis TP, Schonauer MS, Dieckmann CL
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