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Reference: Amutha B, et al. (2008) GTP is required for iron-sulfur cluster biogenesis in mitochondria. J Biol Chem 283(3):1362-71

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Abstract


Iron-sulfur (Fe-S) cluster biogenesis in mitochondria is an essential process and is conserved from yeast to humans. Several proteins with Fe-S cluster cofactors reside in mitochondria, including aconitase [4Fe-4S] and ferredoxin [2Fe-2S]. We found that mitochondria isolated from wild-type yeast contain a pool of apo-aconitase and a machinery capable of forming new clusters and inserting them into this endogenous apo-protein pool. These observations allowed us to develop assays to assess the role of nucleotides (GTP and ATP) in cluster biogenesis in mitochondria. We show that Fe-S cluster biogenesis in isolated mitochondria is enhanced by the addition of GTP and ATP. Hydrolysis of both GTP and ATP is necessary, and addition of ATP cannot circumvent processes that require GTP hydrolysis. Both in vivo and in vitro experiments suggest that GTP must enter into the matrix to exert its effects on cluster biogenesis. Upon import into isolated mitochondria, purified apo-ferredoxin can also be used as a substrate by the Fe-S cluster machinery in a GTP-dependent manner. GTP is likely required for a common step involved in the cluster biogenesis of aconitase and ferredoxin. To our knowledge, this is the first report demonstrating a role of GTP in mitochondrial Fe-S cluster biogenesis.

Reference Type
Journal Article
Authors
Amutha B, Gordon DM, Gu Y, Lyver ER, Dancis A, Pain D
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