The SUN gene family has been defined in S. cerevisiae and comprises a fungal specific family of proteins which show high similarity in their C-terminal domain. Genes of this family are involved in different cellular processes like DNA replication, ageing, mitochondrial biogenesis and cytokinesis. In C. albicans the SUN-family comprises two genes, SUN41 and SIM1. We could demonstrate that C. albicans mutants lacking SUN41 show similar defects as found for S. cerevisiae including defects in cytokinesis. In addition, the SUN41 mutant showed a higher sensitivity towards the cell wall disturbing agent Congo red, whereas no difference was observed in the presence of Calcofluor white. Compared to the wild type SUN41 deletion strains exhibited a defect in biofilm formation, a reduced adherence on a Caco-2 cell monolayer and are unable to form hyphae on solid media under the conditions tested. Interestingly, Sun41p was found to be secreted in the media of cells growing as blastospores as well as hyphae. Our results support a function of SUN41 as glycosidase involved in cytokinesis, cell wall biogenesis, adhesion to host tissue and biofilm formation indicating an important role in host-pathogen interaction.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Annotation Extension||Reference|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Assay||Construct||Conditions||Strain Background||Reference|