Reference: Jeudy S and Schwartz TU (2007) Crystal structure of nucleoporin nic96 reveals a novel, intricate helical domain architecture. J Biol Chem 282(48):34904-12

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Abstract

The nuclear pore complex (NPC) is an elaborate protein machine that mediates macromolecular transport across the nuclear envelope in all eukaryotes. The NPC is formed by nucleoporins that assemble in multiple copies around an eightfold symmetry axis. Homology modeling suggests that most architectural nucleoporins are composed of simple ss-propeller and a-helical repeat domains. Here we present the crystal structure of Nic96, the Nup93 homolog in S. cerevisiae, one of the major components of the NPC. This is the first structure of an a-helical nucleoporin domain. The protein folds into an elongated, mostly a-helical structure. Characteristically non-canonical architectural features define the Nic96 structure. Sequence conservation among Nup93 homologs across all eukaryotes strongly suggests that the distinct topology is evolutionarily well maintained. We propose that the unique Nic96/Nup93 fold has a conserved function in all eukaryotes.

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Journal Article
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Jeudy S, Schwartz TU
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