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Reference: Cheng V, et al. (2007) Genome-Wide Screen for Oxalate-Sensitive Mutants of Saccharomyces cerevisiae. Appl Environ Microbiol 73(18):5919-27

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Abstract


Oxalic acid is an important virulence factor produced by phytopathogenic filamentous fungi. In order to discover yeast genes whose orthologs in the pathogen may confer self-tolerance and whose plant orthologs may protect the host, a Saccharomyces cerevisiae deletion library consisting of 4,827 haploid mutants harboring deletions in non-essential genes was screened for growth inhibition and survival in a rich medium containing 30 mM oxalic acid at pH 3. A total of 31 mutants were identified that had significantly lower cell yields in oxalate medium relative to yields in an oxalate-free medium. About 35% of these mutants had not previously been detected in published screens for sensitivity to sorbic or citric acids. Mutants impaired in endosomal transport, rgp1Delta, ric1Delta, snf7Delta, vps16Delta, vps20Delta, and vps51Delta, were significantly overrepresented relative to their frequency among all verified yeast ORFs. Oxalate exposure to a subset of 5 mutants, drs2Delta, vps16Delta, vps51Delta, ric1Delta, and rib4Delta, was lethal. With the exception of rib4Delta, all of these mutants are impaired in vesicle-mediated transport. Indirect evidence is provided suggesting that the sensitivity of the rib4Delta mutant, a riboflavin auxotroph, is due to oxalate-mediated interference with riboflavin uptake by the putative monocarboxylate transporter Mch5.

Reference Type
Journal Article
Authors
Cheng V, Stotz HU, Hippchen K, Bakalinsky AT
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