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Reference: Nishida Y and Ono BI (2007) An experimental system for the study of mutations in the HMR locus of Saccharomyces cerevisiae: the insertion of Ty into HMRa vs. the conversion of HMRa to HMRalpha. Yeast 24(9):723-30

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Abstract


A cross between a sir4-11 strain (sir4-11 HMLalpha MATalpha HMRa, non-mating type) and an a-mating strain (SIR(+) HMLalpha MATa HMRa) of Saccharomyces cerevisiae forms diploid clones at a frequency of 5 x 10(-6), but the obtained diploid clones often (>70%) have altered forms of the HMRa-containing restriction fragment, designated @ HMRa'. We previously found that some HMRa's are associated with the conversion of HMRa to HMRalpha. In this report, we present evidence that another @ HMRa' associates with the insertion of Ty into Ya of HMR. We also found that the sir4-11 strain increased mating frequency by UV irradiation to a level of 9 x 10(-4), and that generation of HMRa' was completely prevented by disruption of RAD52 of the sir4-11 strain. Hence, we conclude that the mutations that cause generation of HMRa' occur in the sir4-11 strain prior to mating. Due to these mutations, the sir4-11 strain converts to alpha-mating type and readily mates with the a-mating strain. We discuss the usefulness of the sir4-11 strain for the study of mutations in the HMR locus of S. cerevisiae. Copyright (c) 2007 John Wiley & Sons, Ltd.

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Journal Article
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Nishida Y, Ono BI
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