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Reference: Albers E, et al. (2007) Effect of Nutrient Starvation on the Cellular Composition and Metabolic Capacity of Saccharomyces cerevisiae. Appl Environ Microbiol 73(15):4839-48

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Abstract

This investigation addresses the question: What are the important factors for maintenance of a high catabolic capacity under various starvation conditions? S. cerevisiae was cultured in aerobic batch cultures and during the diauxic shift cells were transferred and subjected to 24 hours of starvation. The following conditions were used; carbon starvation, nitrogen starvation in the presence of glucose or ethanol, or dual carbon and nitrogen starvation. During the starvation period changes in biomass composition (including protein, carbohydrate, lipid and nucleic acid content), metabolic activity, sugar transport kinetics and level of selected enzymes were recorded. Subsequent to the starvation period measurement of the remaining catabolic capacity was made by addition of 50 mM glucose. The results showed that the glucose transport capacity is a key factor for maintenance of a high metabolic capacity in many, but not all cases. Cells starved of carbon, carbon and nitrogen or nitrogen in the presence of glucose all indicated that the metabolic capacity was indeed controlled by the glucose transport ability perhaps with some influence also of hexokinase, phosphofructokinase, aldolase and enolase levels. However, it was also demonstrated that there is no such correlation when nitrogen starvation is performed in the presence of ethanol instead of glucose.

Reference Type
Journal Article
Authors
Albers E, Larsson C, Andlid T, Walsh MC, Gustafsson L
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