Reference: Gipson CL, et al. (2007) Functional characterization of yeast telomerase RNA dimerization. J Biol Chem 282(26):18857-63

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Abstract


Telomerase is the cellular RNA-dependent DNA polymerase (i.e., reverse transcriptase) that uses an integral RNA template to synthesize telomeric DNA repeats at the ends of linear chromosomes. Human telomerase RNA (hTERC) is thought to function as a dimeric complex, consisting of two hTERC RNAs that interact with each other physically as well as genetically. We show here for the first time that the yeast S. cerevisiae telomerase TLC1 RNA likewise forms dimers in vitro. TLC1 dimerization depends on a unique 6-base self-complementary sequence, which closely mimics palindromic sequences that mediate functional dimerization of the HIV-1 and other retroviral genomes. We find that dissimilar, but comparably located, TLC1 palindromes from other sensu stricto yeasts can functionally substitute for that of S. cerevisiae. Yeast cells expressing dimerization-defective TLC1 alleles have shorter telomeres than those with wildtype TLC1. This study, therefore, highlights dimerization as a functionally conserved feature of the RNA templates utilized by reverse transcriptases of both viral and cellular origins.

Reference Type
Journal Article
Authors
Gipson CL, Xin ZT, Danzy SC, Parslow TG, Ly H
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