Take our Survey

Reference: Liang F and Wang Y (2007) DNA damage checkpoints inhibit mitotic exit by two different mechanisms. Mol Cell Biol 27(14):5067-78

Reference Help

Abstract

Cyclin dependent kinase (Cdk) governs cell cycle progression and its kinase activity fluctuates during cell cycle. Mitotic exit pathways are responsible for the inactivation of Cdk after chromosome segregation by promoting the release of a nucleolar sequestered phosphatase Cdc14, which antagonizes Cdk. In the budding yeast Saccharomyces cerevisiae, mitotic exit is controlled by FEAR (Cdc fourteen early anaphase release) and MEN (mitotic exit network) pathways. In response to DNA damage, two branches of the DNA damage checkpoint, Chk1 and Rad53, are activated in budding yeast to prevent anaphase entry and mitotic exit, allowing cells more time to repair damaged DNA. Here we present evidence indicating that yeast cells negatively regulate mitotic exit through two distinct pathways in response to DNA damage. Rad53 prevents mitotic exit by inhibiting MEN pathway, whereas Chk1 pathway prevents FEAR pathway dependent Cdc14 release in the presence of DNA damage. In contrary to previous data, Rad53 pathway negatively regulates MEN independently of Cdc5, a polo-like kinase essential for mitotic exit. Instead, defective Rad53 pathway alleviates the inhibition of MEN by Bfa1.

Reference Type
Journal Article
Authors
Liang F, Wang Y
Primary Lit For
Additional Lit For
Review For

Interaction Annotations

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference