Reference: Hurto RL, et al. (2007) Inorganic Phosphate Deprivation Causes tRNA Nuclear Accumulation via Retrograde Transport in Saccharomyces cerevisiae. Genetics 176(2):841-52

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Abstract


Nuclear export of tRNA is an essential eukaryotic function, yet the one known yeast tRNA nuclear exporter, Los1, is unessential. Moreover recent studies have shown that tRNAs can move retrograde from the cytosol to the nucleus by an undefined process. Therefore, additional gene products involved in tRNA nucleus-cytosol dynamics have yet to be identified. Synthetic genetic analysis was employed to identify proteins involved in Los1-independent tRNA transport and in regulating tRNA nuclear-cytosol distribution. These studies uncovered synthetic interactions between los1Delta and pho88Delta, involved in inorganic phopshate up-take. Futher analysis revealed that inorganic phopsphate deprivation causes transient, temperature-dependent nuclear accumulation of mature, cytomplasmic tRNA within nuclei via a Mtr10- and retrograde-dependent pathway, providing a novel connection between tRNA subcellular dynamics and phosphate availability.

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Journal Article
Authors
Hurto RL, Tong AH, Boone C, Hopper AK
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