Reference: Anderson VE, et al. (2007) Novel sfi1 Alleles Uncover Additional Functions for Sfi1p in Bipolar Spindle Assembly and Function. Mol Biol Cell 18(6):2047-56

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Abstract


Monitoring Editor: Tim Stearns A variety of spindle and kinetochore defects have been shown to induce a mitotic delay through activation of the spindle checkpoint. With the aim of identifying novel mitotic defects we carried out a mad1 synthetic lethal screen in budding yeast. In this screen four novel alleles of sfi1 were isolated. SFI1 is an essential gene, previously identified through its interaction with centrin/CDC31 and shown to be required for spindle pole body (SPB) duplication. The new mutations were all found in the C-terminal domain of Sfi1p, which has no known function but is well conserved among budding yeasts. Analysis of the novel sfi1 mutants, through a combination of light and electron microscopy, revealed duplicated SPBs <0.3 microm apart. Importantly, these SPBs have completed duplication but are not separated, suggesting a possible defect in splitting of the bridge. We discuss possible roles for Sfi1p in this step in bipolar spindle assembly.

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Journal Article
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Anderson VE, Prudden J, Prochnik S, Giddings Jr TH, Hardwick KG
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