Reference: Hook BA, et al. (2007) Two yeast PUF proteins negatively regulate a single mRNA. J Biol Chem 282(21):15430-8

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Abstract


mRNA stability and translation are regulated by 3'UTR binding repressor proteins. PUF proteins provide a paradigm for these regulatory molecules: like other repressors, they inhibit translation, enhance mRNA decay, and promote poly(A) removal. Here we show that a single mRNA in Saccharomyces cerevisiae, encoding the HO endonuclease, is regulated by two distinct PUF proteins, Puf4p and Mpt5p. These proteins bind to adjacent sites and can co-occupy the mRNA. Both proteins are required for full repression and deadenylation in vivo; their removal dramatically stabilizes the mRNA. The two proteins act through overlapping but non-identical mechanisms: repression by Puf4p is dependent on deadenylation, while repression by Mpt5p can occur through additional mechanisms. Combinatorial action of the two regulatory proteins may allow responses to specific environmental cues, and be common in 3'UTR-mediated control.

Reference Type
Journal Article
Authors
Hook BA, Goldstrohm AC, Seay DJ, Wickens M
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