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Reference: Levens D, et al. (1981) Purification of mitochondrial RNA polymerase from Saccharomyces cerevisiae. J Biol Chem 256(3):1474-81

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Abstract

The RNA polymerase from the mitochondria of Saccharomyces cerevisiae has been extensively purified by Sepharose 4B, heparin Sepharose 4B phosphocellulose, and DEAE-Sephadex A-50 chromatography. The activity co-sediments with a 45,000-dalton polypeptide at 6.3 S in glycerol gradients. The activity is inhibited by antibodies to the 45,000-dalton polypeptide. The activity is not inhibited by rifampicin or alpha-amanitin. It requires Mg2+ and is inhibited by elevated ionic strength and Mn2+. The most efficient template for the RNA polymerase is poly[d(AT)], with mtDNA being the preferred natural template. The RNA polymerase transcribes mtDNA from the petite strain F11 in a nonrandom manner.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Levens D, Lustig A, Rabinowitz M
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