The glucose sensors Gpr1, Snf3 and Rgt2 generate the earliest signals produced by glucose in yeast. We showed that a lack of Gpr1 or Snf3/Rgt2 decreased by twofold the glucose induction of SUC2, but had no effect on the induction of pyruvate decarboxylase (Pdc). The induction of HXT1 was not affected by the absence of Gpr1. In an hxk1 hxk2 glk1 strain, high glucose fully induced SUC2, caused partial induction of HXT1 and had no effect on Pdc. In this strain, SUC2 induction was dependent on Gpr1, but HXT1 induction was not. Hxk2, required for the high expression of HXT1, was dispensable for the full induction of SUC2 or Pdc. These results indicate that glucose does not induce transcription through a single signalling pathway, but that several pathways may, in different combinations, regulate the transcription of different genes.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Annotation Extension||Reference|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Assay||Construct||Conditions||Strain Background||Reference|