Strains of Saccharomyces cerevisiae carrying a 1.4 x 10(6) dalton double-stranded (ds) RNA in virus-like particles (the killer plasmid or virus) secrete a toxin that is lethal to strains not carrying this plasmid (virus). The mak10 gene is one of 24 chromosomal genes (called pets, mak1, mak2,...) that are needed to maintain and replicate the killer plasmid. We report here isolation of spontaneous and induced mutants in which the killer plasmid is maintained and replicated in spite of a defect in the mak10 gene. The bypass (or suppressor) mutations in these strains are in the mitochondrial genome. Respiratory deficiency produced by various chromosomal pet mutations, by chloramphenicol, or by antimycin A, does not bypass the mak10-1 mutation. Several spontaneous mak10-1 killer strains have about 12-fold more of the killer plasmid ds RNA than do wild-type killers. Although the absence of mitochondrial DNA bypasses mak10-1, it does not bypass pets-1, mak1-1, mak3-1, mak4-1, mak5-1, mak6-1, mak7-1, or mak8-1.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Annotation Extension||Reference|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Assay||Construct||Conditions||Strain Background||Reference|