Reference: Lockshon D, et al. (2007) The sensitivity of yeast mutants to oleic Acid implicates the peroxisome and other processes in membrane function. Genetics 175(1):77-91

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Abstract

The peroxisome, sole site of beta-oxidation in Saccharomyces cerevisiae, is known to be required for optimal growth in the presence of fatty acid. Screening the haploid yeast deletion collection identified ~130 genes, 23 encoding peroxisomal proteins, necessary for normal growth on oleic acid. Oleate slightly enhances growth of wild-type yeast and inhibits growth of all strains identified by the screen. Non-peroxisomal processes, among them chromatin modification by H2AZ, Pol II mediator function, and cell wall-associated activities, also prevent oleate toxicity. The most oleate-inhibited strains lack Sap190, a putative adaptor for the PP2A-type protein phosphatase Sit4 (that is also required for normal growth on oleate) and Ilm1, a protein of unknown function. Palmitoleate, the other main unsaturated fatty acid of Saccharomyces, fails to inhibit growth of the sap190Delta, sit4Delta and ilm1Deltastrains. Data is presented that suggests that oleate inhibition of the growth of a peroxisomal mutant is due to an increase in plasma membrane porosity. We propose that yeast deficient in peroxisomal and other functions are sensitive to oleate perhaps because of an inability to effectively control the fatty acid composition of membrane phospholipid.

Reference Type
Journal Article
Authors
Lockshon D, Surface LE, Kerr EO, Kaeberlein M, Kennedy B
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