The mutant 263-H9 with hypersensitivity to several stress conditions (1.5 mol/L Sorbitol, 0.65 mol/L NaCl and 15) was obtained by using transposon mutagenesis in the Saccharomyces cerevisiae strain W303-1A. Unlike other mutants the transposon in 263-H9 was intergenic between GIP2 and YER053C-A. Using gene knockout, a yeast genomic library and other methods, the gene correlated with the salt stress response was identified. The data indicated that the phenotype of 263-H9 was not directly caused by the insertion of the transposon. On the other hand, the hypersensitivity to salt and other stress conditions was due to the deletion of 5 base pairs close to position 936bp in the PBS2 gene essential for HOG signal pathway regulation under salt stress.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|