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Reference: Yu DK, et al. (2006) [A SCAR Marker for Resistance to Aspergillus flavus in Peanut (Arachis hypogaea L.).] Yi Chuan 28(10):1294-1298

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Abstract


The mutant 263-H9 with hypersensitivity to several stress conditions (1.5 mol/L Sorbitol, 0.65 mol/L NaCl and 15) was obtained by using transposon mutagenesis in the Saccharomyces cerevisiae strain W303-1A. Unlike other mutants the transposon in 263-H9 was intergenic between GIP2 and YER053C-A. Using gene knockout, a yeast genomic library and other methods, the gene correlated with the salt stress response was identified. The data indicated that the phenotype of 263-H9 was not directly caused by the insertion of the transposon. On the other hand, the hypersensitivity to salt and other stress conditions was due to the deletion of 5 base pairs close to position 936bp in the PBS2 gene essential for HOG signal pathway regulation under salt stress.

Reference Type
Journal Article
Authors
Yu DK, Zhang XH, Liu XY, Bao XM, Gao D
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