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Reference: Hedges J, et al. (2006) Mapping the functional domains of yeast NMD3, the nuclear export adapter for the 60 S ribosomal subunit. J Biol Chem 281(48):36579-87

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Abstract

Nuclear export of the large ribosomal subunit requires the adapter protein Nmd3p to provide a leucine-rich nuclear export signal (NES) that is recognized by the export receptor Crm1. Nmd3p binds to the pre-60S subunit in the nucleus. After export to the cytoplasm, the release of Nmd3p depends on the ribosomal protein Rpl10p and the GTPase Lsg1p. Here, we have carried out a mutational analysis of Nmd3 to better define the domains responsible for nucleocytoplasmic shuttling and ribosome binding. We show that mutations in two regions of Nmd3p affect 60S binding, suggesting that its binding to the subunit is multivalent.

Reference Type
Journal Article
Authors
Hedges J, Chen YI, West M, Bussiere C, Johnson AW
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