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Reference: Dieppois G, et al. (2006) Cotranscriptional recruitment to the mRNA export receptor mex67p contributes to nuclear pore anchoring of activated genes. Mol Cell Biol 26(21):7858-70

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Abstract

Transcription activation of some yeast genes is paralleled by their repositioning to the nuclear periphery, but the mechanism underlying gene anchoring is poorly defined. We show that NPC-associated Mlp1p and the shuttling mRNA export receptor Mex67p contribute to the stable association of the activated GAL10 and HSP104 genes with the nuclear periphery. However, we find no obligatory link between gene positioning and gene expression. Furthermore, gene anchoring correlates with the co-transcriptional recruitment of Mex67p to transcribing genes. Notably, the association of Mex67p with chromatin is not mediated by RNA. Interestingly, a mutant GAL2 gene lacking the coding region is still able to recruit Mex67p upon transcriptional activation and to relocate to the nuclear periphery. Together these data suggest that, at least for GAL2, nascent mRNP does not play a major role in gene anchoring and that the early recruitment of Mex67p contributes to gene repositioning by virtue of an RNA independent process.

Reference Type
Journal Article
Authors
Dieppois G, Iglesias N, Stutz F
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