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Reference: Zybailov B, et al. (2006) Statistical Analysis of Membrane Proteome Expression Changes in Saccharomyces cerevisiae. J Proteome Res 5(9):2339-47

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Abstract


We have devised an approach for analyzing shotgun proteomics datasets based on the normalized spectral abundance factor that can be used for quantitative proteomics analysis. Three biological replicates of samples enriched for plasma membranes were isolated from S. cerevisiae grown in (14)N-rich media and (15)N-minimal media and analyzed via quantitative multidimensional protein identification technology. The natural log transformation of NSAF values from S. cerevisiae cells grown in (14)N YPD media and (15)N-minimal media had a normal distribution. The t-test analysis demonstrated 221 of 1316 proteins were significantly overexpressed in one or the other growth conditions with a p value <0.05. Notably, amino acid transporters were among the 14 membrane proteins that were significantly upregulated in cells grown in minimal media, and we functionally validated these increases in protein expression with radioisotope uptake assays for selected proteins.

Reference Type
Journal Article
Authors
Zybailov B, Mosley AL, Sardiu ME, Coleman MK, Florens L, Washburn MP
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