GTS1 induces flocculation when overexpressed in the Saccharomyces cerevisiae strain W303-1A, which carries a mutant FLO8, the activator of flocculin genes. Herein, we report that the GTS1-induced flocculation was flocculin-dependent in nature and was caused by expression of the major flocculin Flo1p. Gts1p bound to the repressor Sfl1p, and their interaction at the transcriptional level was shown by reporter gene assays using the FLO1 promoter, suggesting that Gts1p induces the expression of FLO1 by inhibiting Sfl1p. Furthermore, the Q-rich domain with the preceding 18 amino acids of Gts1p bound mediators for RNA polymerase II.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|