Take our Survey

Reference: Marques JM, et al. (2006) Saccharomyces cerevisiae Hog1 protein phosphorylation upon exposure to bacterial endotoxin. J Biol Chem 281(34):24687-94

Reference Help

Abstract

The yeast Hog1 protein is both functionally and structurally similar to the mammalian p38, belonging to the same family of MAP kinases and responding to extracellular changes in osmolarity. Since p38 mediates LPS effects in mammalian cells, we now tested the responsiveness of Hog1 upon exposure of the yeast S. cerevisiae to bacterial LPS. In the presence of E. coli LPS (100 ng/mL) and to an endotoxically active, hexaacylated, synthetic lipid A (compound 506; 100 ng/mL), Hog1 becomes phosphorylated with a maximum of phosphorylation between 3-6 hours, whereas a tetraacylated, inactive form of lipid A (compound 406) did not cause any modification in the phosphorylation state of Hog1. A triple labelling immunocytochemical study showed that phosphorylated Hog1 translocates into the nucleus after 90 min incubation and becomes sparsely located in the cytoplasm. The translocation of the phospho-Hog1 is preceded by an increased expression of the HOG1 gene and concomitant with the expression of the Hog1 target gene, GPD1. We also observed that cells unable to synthesise Hog1 do not resist to LPS as efficiently as wild-type cells. We conclude that the yeast S. cerevisiae is able to respond to the presence of gram-negative bacteria endotoxin, and that Hog1 is involved in this response.

Reference Type
Journal Article
Authors
Marques JM, Rodrigues RJ, de Magalhaes-Sant'ana AC, Goncalves T
Primary Lit For
Additional Lit For
Review For

Interaction Annotations

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference