The assembly of the yeast transcription factors IIIA, IIIB, and IIIC, represented by crude chromatographic fractions, into a stable complex on the 5 S RNA gene has been assessed using a template competition assay (Bogenhagen, D. F., Wormington, W. M., and Brown, D. D. (1982) Cell 28, 413-421). The yeast factor A can interact transiently with the yeast 5 S RNA gene, but both factors A and C are required for the formation of a stable complex. Factor A, but not factor C, is sequestered into this complex. Both factors A and C, however, are required for the sequestration of factor B into the 5 S RNA gene transcription complex. During this study, it was noted that an amount of factor C which was insufficient to lead to significant transcription of the 5 S RNA gene could nonetheless efficiently stabilize factor A on the template. This suggests that factor C may initially act catalytically to stabilize a transient interaction of factor A with the 5 S RNA gene before assuming a stoichiometric role in the assembly of the complete transcription complex. Alternative interpretations of this observation are discussed.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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