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Reference: Yokoyama H, et al. (2006) Involvement of calcineurin-dependent degradation of Yap1p in Ca(2+)-induced G(2) cell-cycle regulation in Saccharomyces cerevisiae. EMBO Rep 7(5):519-24

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Abstract

The Ca(2+)-activated pathways in Saccharomyces cerevisiae induce a delay in the onset of mitosis through the activation of Swe1p, a negative regulatory kinase that inhibits the Cdc28p/Clb complex. We isolated the YAP1 gene as a multicopy suppressor of calcium sensitivity owing to the loss of ZDS1, a negative regulator of SWE1 and CLN2 gene expression. YAP1 deletion on a zds1Delta background exacerbated the Ca(2+)-related phenotype. Yap1p was degraded in a calcineurin-dependent manner when cells were exposed to calcium. In yap1Delta cells, the expression level of the RPN4 gene encoding a transcription factor for the subunits of the ubiquitin-proteasome system was diminished. The deletion of YAP1 gene or RPN4 gene led to the accumulation of Swe1p and Cln2p. Yap1p was a substrate of calcineurin in vivo and in vitro. The calcineurin-mediated Yap1p degradation seems to be a long adaptive response that assures a G(2) delay in response to a stress that causes the activation of the calcium signalling pathways.

Reference Type
Journal Article
Authors
Yokoyama H, Mizunuma M, Okamoto M, Yamamoto J, Hirata D, Miyakawa T
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