Take our Survey

Reference: Welch JW, et al. (1987) Synaptic relations in meiotic gene conversion at the iterated CUP1r locus of S. cerevisiae. Experientia Suppl 52:431-7

Reference Help

Abstract


This study concerns a comparative molecular analysis of copy number changes in two hybrids that differ in the extent of homologies at the CUP1r locus. Hybrid JW1020 is a diploid wherein each parent contributed nine identical, tandemly arrayed 2.0 kb repeat units. Genomic DNA was isolated from each of the spore colonies in a sample of 200 unselected tetrads. About 15% displayed copy number changes, i.e., increases or decreases of one or more complete units. Changes on a per tetrad basis occurred as often in a single spore colony as changes in each of two spores. Such double changes are rarely reciprocal in character. To account for the observed qualitative and quantitative copy number shifts, we propose a molecular recombination model that posits partial, incomplete synaptic pairing and gene conversion of the unpaired regions with or without associated crossing over. A second contrasting study centers on the copy number alterations and recombinational events uncovered in a molecular analysis of 50 unselected tetrads generated by hybrid EB8. Unlike the hybrid JW1020, the EB8 diploid strain carried a six copy tandem array of 1.1 kb units at the CUP1r locus in one parental homologue and a five copy array of 1.6 kb units at the corresponding chromosome VIII locus. These natural alleles were recovered from industrial yeast strains by conventional genetic procedures and characterized by restrictional analysis. Twelve tetrads exhibit evidence for several different types of recombination events. However,ordinary crossover exchanges are conspicuously absent. We suppose that the repetitious nonhomologies generate DNA configurations sufficient to disrupt the effective synapsis over the entire locus.(ABSTRACT TRUNCATED AT 250 WORDS.

Reference Type
Journal Article
Authors
Welch JW, Maloney DH, Fogel S
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference