Reference: Korkegian A, et al. (2005) Computational thermostabilization of an enzyme. Science 308(5723):857-60

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Abstract


Thermostabilizing an enzyme while maintaining its activity for industrial or biomedical applications can be difficult with traditional selection methods. We describe a rapid computational approach that identified three mutations within a model enzyme that produced a 10 degrees C increase in apparent melting temperature T(m) and a 30-fold increase in half-life at 50 degrees C, with no reduction in catalytic efficiency. The effects of the mutations were synergistic, giving an increase in excess of the sum of their individual effects. The redesigned enzyme induced an increased, temperature-dependent bacterial growth rate under conditions that required its activity, thereby coupling molecular and metabolic engineering.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Korkegian A, Black ME, Baker D, Stoddard BL
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