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Reference: Bonner JJ, et al. (2000) Structural analysis of yeast HSF by site-specific crosslinking. J Mol Biol 302(3):581-92

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Abstract


We have introduced cysteine substitutions into the yeast HSF1 gene at a variety of locations. Most have no phenotypic effect, and therefore provide site-specific probes for thiol-specific reagents. Crosslinking of single mutants identifies locations where equivalent regions of individual monomers can approach each other in the HSF trimer. Crosslinking of double mutants indicates regions that can approach closely within a single subunit. Results for the DNA binding domain and trimerization domain are consistent with known structural information, and provide essential controls on the validity of the technique. In contrast to these two domains, the N-terminal and C-terminal domains, wherein lie the transcriptional activators, are highly flexible, and do not appear to be in stable contact with any other portions of the protein. None of these patterns are affected by the conformational change that is induced by superoxide or heat shock. We suggest a new model for the mechanism of HSF regulation that accomodates the structural information provided by these studies.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Bonner JJ, Chen D, Storey K, Tushan M, Lea K
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