In Saccharomycopsis (Candida) lipolytica direct cysteine synthesis is mediated by two different, simultaneously acting cysteine synthases: a low molecular mass (74,000 daltons) monofunctional enzyme (Km for O-acetyl-l-serine 23.5 mm) and a high molecular mass (220,000 daltons) bifunctional cysteine-homocysteine synthase (Km for O-acetyl-l-serine 55.0 mm). The latter enzyme is also involved in direct homocysteine synthesis from O-acetyl-l-homoserine (Km 11.9 mm). Evidence is presented that a fraction of homocysteine can be synthesized from cysteine via cystathionine. Our previous results (Morzycka & Paszewski, FEBS Lett., 1979, 101, 97-100; Mol. Gen. Genet., 1979, 174, 33-38) together with the data presented here strongly suggest that both S., lipolytica synthases are under control of the same regulatory system, together with the other enzymes of sulphur metabolism: ATP-sulphurylase and aryl-sulphatase. gamma-Cystathionase, an enzyme involved in cysteine synthesis from homocysteine, is controlled exclusively by cysteine.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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