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Reference: Van Ryk DI and Nazar RN (1992) Effect of sequence mutations on the higher order structure of the yeast 5 S rRNA. J Mol Biol 226(4):1027-35

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Abstract

Mutant yeast ribosomal 5 S RNAs were probed by enzymatic cleavage and chemical reactivity to define further the higher order structure. Mutations that destabilized helix IV resulted in an altered tertiary structure in which a reduced reactivity to ethylnitrosourea at U90 and G91 could be correlated with greater enzymatic and Fe(II)-EDTA cleavages in helices II and V. The results provide direct evidence for, and a further definition of, a structural juxtaposition between helix II and the end of helix IV and indicate that, in contrast to earlier suggestions, the remaining tertiary structure is sufficiently stable to prevent "pseudoknot-like" interactions between helices III and IV. The data are fully consistent with the "lollipop" model of the tertiary structure.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Comparative Study
Authors
Van Ryk DI, Nazar RN
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