Reference: Guadalupe Cabral M, et al. (2004) Adaptative responses in yeast to the herbicide 2-methyl-4-chlorophenoxyacetic acid at the level of intracellular pH homeostasis. J Appl Microbiol 96(3):603-12

Reference Help

Abstract


OBJECTIVE: The objective of this work was to examine adaptative responses occurring in Saccharomyces cerevisiae following exposure to the herbicide 2-methyl-4-chlorophenoxyacetic acid (MCPA). RESULTS: The exposure of a yeast cell population to MCPA concentrations of moderate toxicity led to a period of latency before eventual resumption of inhibited growth. During this period of adaptation, the plasma membrane (PM) H+-ATPase was activated, in coordination with the decrease of intracellular pH (pHi), cell viability and average cell volume. The in vivo activation of this ATPase was demonstrated either by assaying PM-ATPase activity in membrane suspensions extracted from cells grown in the presence or absence of MCPA or by measuring the in vivo H+-pumping activity in the same cells. The PM-H+-ATPase activation could not be attributed to transcriptional activation of the encoding genes PMA1 and PMA2. CONCLUSIONS: The activity of PM-H+-ATPase was stimulated and the internal cell volume decreased during yeast adaptation to growth under MCPA stress. Based on the values estimated for the pHi, we hypothesize that these cell responses may contribute to the restoration of pHi homeostasis during recovery from MCPA stress. CONCLUSIONS: This work is a contribution to the understanding of the toxic effects of the herbicide MCPA and of physiological mechanisms underlying adaptation to MCPA, in the eukaryotic model S. cerevisiae. Results may be useful to elucidate the adaptation mechanisms to this xenobiotic compound in more complex and experimentally less-accessible eukaryotes. They also provide indications to assist the use of yeast cells as a bioassay system to assess the toxicity of phenoxyacetic acid herbicides and of other lipophilic xenobiotics, aiming at reducing the use of animals in toxicity testing.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Guadalupe Cabral M, Sa-Correia I, Viegas CA
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference