In yeast, several membrane-bound nutrient transporters have been shown to be regulated by the covalent attachment of ubiquitin, a signal for internalization and degradation. The yeast gene DOT4 encodes one of a family of enzymes which remove ubiquitin from proteins to which the peptide has been attached. Mutations in DOT4 cause a growth defect that is particularly severe when combined with mutations in nutrient biosynthetic enzymes (1). These results suggest that nutrient transport or utilization may be compromised in dot4 mutants. We now report that preventing the down-regulation by endocytosis of membrane proteins partially suppressed the dot4Delta growth defect. We also show that the activity of the amino acid permease Gap1p is reduced in DOT4 mutants. This correlates with a reduction in Gap1 protein level, while GAP1 mRNA levels remains unchanged. We conclude that Dot4p is involved in posttranscriptionally regulating Gap1p, and possibly other transporters as well.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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