Reference: Kodaki T, et al. (2003) Differential transcriptional regulation of two distinct S-adenosylmethionine synthetase genes (SAM1 and SAM2) of Saccharomyces cerevisiae. Nucleic Acids Res Suppl (3):303-4

Reference Help

Abstract


Expression of a number of genes encoding enzymes involved in phospholipid biosynthesis in yeast Saccharomyces cerevisiae is known to be repressed on the addition of myo-inositol and choline to the culture medium (inositol-choline regulation). All genes subject to this inositol-choline regulation have an octamer sequence 5'-CATRTGAA-3' in their upstream regions and those octamer sequences play an important role in this regulation. To confirm the role of the octamer sequence further, we studied the transcriptional regulation of two distinct S-adenosylmethionine synthetase genes (SAM1 and SAM2) of S. cerevisiae. Quantitative RT-PCR analysis showed that only the SAM2 gene was subject to the inositol-choline regulation, consistent with the fact that only the SAM2 gene has two octamer sequences in its upstream region. Furthermore, functional promoter analysis revealed that the proximal octamer sequence of the SAM2 gene has an essential role for this regulation.

Reference Type
Journal Article
Authors
Kodaki T, Tsuji S, Otani N, Yamamoto D, Rao KS, Watanabe S, Tsukatsune M, Makino K
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference