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Reference: Xu R, et al. (2002) Production of meiosis-activating sterols from metabolically engineered yeast. J Am Chem Soc 124(6):918-9

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Abstract


Meiosis-activating sterols (MAS), a class of potent regulators of reproductive processes, are difficult to obtain by chemical synthesis or isolation from natural sources. We demonstrate the development of metabolically engineered strains of Saccharomyces cerevisiae that accumulate MAS as the predominant sterol product. Homologous recombination was used to construct an erg24[Delta] erg25[Delta] hem1[Delta] mutant RXY4.3, which lacked sterol [Delta](14) reductase, C-4 oxidase, and [delta]-aminolevulinate synthase. The HEM1 deletion allowed sterol import and rendered RXY4.3 viable under aerobic conditions. This mutant accumulated 4,4-dimethyl-5[alpha]-cholesta-8,14,24-trien-3[beta]-ol (FF-MAS), and a similar erg25[Delta] hem1[Delta] mutant produced 4,4-dimethyl-5[alpha]-cholesta-8,24-dien-3[beta]-ol (T-MAS). Based on consistent yields of [similar]5 [mu]g of FF-MAS per mL of culture, fermentation of genetically modified yeast compares favorably with other approaches to produce MAS.

Reference Type
Journal Article
Authors
Xu R, Wilson WK, Matsuda SP
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