Reference: Engel M, et al. (1994) Phosphorylation of nm23/nucleoside diphosphate kinase by casein kinase 2 in vitro. Biochem Biophys Res Commun 199(2):1041-8

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Abstract


We have investigated phosphorylation of human nucleoside diphosphate kinase (NDPK) and of homologous NDPK from different species by human casein kinase 2 (CK-2). The human NDPK isotypes A and B were phosphorylated by CK-2 in vitro both when the purified proteins and total lysate of HL-60 leukemia cells were used. The homologous NDPK's from Yeast and E. coli were also substrates for CK-2 in vitro, but not Drosophila NDPK. Phosphorylation of all NDPK types by the CK-2 holoenzyme was entirely polyamine-dependent. The CK-2 phosphorylation site in human NDPK A, that was about 2.5 times stronger phosphorylated than was the B isotype, was tentatively assigned to Ser-122. The location of the corresponding residue in the 3D-structure of the 80% homologous Drosophila NDPK suggests that its phosphorylation may directly influence substrate binding and/or catalysis.

Reference Type
Comparative Study | Journal Article
Authors
Engel M, Issinger OG, Lascu I, Seib T, Dooley S, Zang KD, Welter C
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