Take our Survey

Reference: Chu FK, et al. (1978) The effect of carbohydrate depletion on the properties of yeast external invertase. J Biol Chem 253(24):8691-3

Reference Help

Abstract


To study the role of carbohydrate on the properties of the external invertase of Saccharomyces cerevisiae, about 90% of the carbohydrate associated with this glycoprotein was removed by endo-beta-N-acetylglucosaminidase H. Although the catalytic and physical properties of the carbohydrate-depleted (CHO(-)) and the carbohydrate-containing (CHO(+)) enzyme did not differ significantly in a number of parameters tested, the CHO(-) form was much less stable to multiple freezethaw treatment, incubation at 5O C, acidic conditions, and trypsin digestion. In addition, following guanidine hydrochloride inactivation of CHO(+) and CHO(-) enzyme, a marked difference in the recovery of enzyme activity was noted on removal of this denaturant; 76% of the original activity was restored for native invertase, but only 34% for CHO(-) invertase. Circular dichroism studies of the renatured forms and their parent molecules revealed significant differences in ellipticity at 232 and 293 nm with renatured CHO(-) invertase. Similarly, tryptophan fluorescence studies showed a significant reduction in fluorescence in the renatured CHO(-) enzyme. Incubation of the released oligosaccharides with this form of the enzyme during or after guanidine hydrochloride treatment did not increase the amount of enzyme activity recovered. From these studies, it would appear that the covalently bound carbohydrate of yeast invertase plays a role in promoting the folding of the enzyme protein to its most stable conformation.

Reference Type
Journal Article
Authors
Chu FK, Trimble RB, Maley F
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference