Reference: Lin SX, et al. (1984) Fast kinetic study of yeast phenylalanyl-tRNA synthetase: role of tRNAPhe in the discrimination between tyrosine and phenylalanine. Biochemistry 23(18):4109-16

Reference Help

Abstract


An extensive study of the discrimination between phenylalanine and tyrosine by yeast phenylalanyl-tRNA synthetase was carried out in the presence of native tRNAPhe. Our results clearly show that, besides the previously reported dissociation of tyrosyl adenylate from the enzyme template [Lin, S. X., Baltzinger, M., & Remy, P. (1983) Biochemistry 22, 681-689], two more correction processes are involved in the rejection of tyrosine in the presence of tRNAPhe. A minor part of the misactivated tyrosine is indeed transferred to tRNAPhe, but the resulting misaminoacylated tRNA is very rapidly hydrolyzed (kh approximately equal to 60 s-1), as it has already been shown for other systems. However, the major part of the misactivated tyrosine is rejected as the result of a pretransfer correction consisting of the fast hydrolysis (k'h approximately equal to 20 s-1) of the enzyme-bound noncognate adenylate induced by the binding of native tRNAPhe. The transfer step itself is found to be non-specific, as the rate constant is almost the same for phenylalanine and tyrosine. This result is supported by the observation that tyrosine and phenylalanine are also transferred at the same rate to tRNAPheox-red. It is shown that the integrity of the 3'-terminal adenosine of the tRNA is critical for triggering the pretransfer hydrolysis of enzyme-bound noncognate aminoacyl adenylate. A detailed kinetic analysis is presented that shows that the observed rate constant of tRNAPhe tyrosylation and the rate of disappearance of enzyme-tyrosyl adenylate complex are in fact apparent rate constants.(ABSTRACT TRUNCATED AT 250 WORDS)

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Lin SX, Baltzinger M, Remy P
Primary Lit For
Additional Lit For
Review For

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene/Complex Qualifier Gene Ontology Term Aspect Annotation Extension Evidence Method Source Assigned On Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Disease Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Disease Ontology Term Qualifier Evidence Method Source Assigned On Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Direction Regulation Of Happens During Method Evidence

Post-translational Modifications


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Site Modification Modifier Reference

Interaction Annotations


Genetic Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Assay Annotation Action Modification Source Reference

Functional Complementation Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Species Gene ID Strain background Direction Details Source Reference