Take our Survey

Reference: Kobayashi K and Yoshimoto A (1982) Studies on yeast sulfite reductase. VI. Use of the effects of ionic strength as a probe for enzyme structure and mechanism. Biochim Biophys Acta 709(1):46-52

Reference Help

Abstract


The effects of ionic strength on the structure of yeast sulfite reductase (EC 1.8.1.2) were investigated. Gel filtration and sedimentation analysis revealed that this enzyme dissociated at ionic strength below 0.1 into two components having molecular weights of 300000 and sedimentation coefficients of 7-9 S. The secondary structure and the surroundings of the prosthetic groups were not appreciably affected by ionic strength. This enzyme showed CD spectra in the near-ultraviolet region considered to be due to tyrosine residues. On lowering the ionic strength, they decreased, together with the disappearance of the difference spectra in the same region, indicating that some tyrosine residues became exposed to the environment at low ionic strength. SH groups were also exposed at low ionic strength and reacted with DTNB and PCMB more easily to cause the loss of NADPH-sulfite reductase activity. These essential SH groups as well as some tyrosine residues may exist in the region of contact between the two components constituting the enzyme molecule.

Reference Type
Journal Article
Authors
Kobayashi K, Yoshimoto A
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference