We describe techniques for production and chromatographic fractionation of a transcriptionally active whole-cell extract from Saccharomyces cerevisiae. The procedure is suitable for large-scale isolation of the factors involved in mRNA synthesis. Both yeast transcription factor IIB and TATA-binding protein were purified from the extract as single species using an immunoblot assay. In addition, the three previously described isoforms of yeast RNA polymerase II were resolved and form IIa, the intact, unphosphorylated isoform proposed to be involved in initiation, was purified to apparent homogeneity.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|