In Saccharomyces cerevisiae, the suggested functions of DNA polymerases (DNApol) have been based primarily on the characterization of the wild-type and mutant enzymes via in vitro studies. Here we describe a novel replication system to decipher the role of different DNA polymerases in in vivo DNA replication. Using this system, [alpha-32P]dNTP is allowed to cross the membrane of permeabilized cells; then the nature of the radiolabeled products of DNA synthesis is analyzed by gel electrophoresis and densitometry. Results of such analyses show that these replication intermediates are synthesized in the range 50-1,300 bp, which are then rapidly elongated and then ligated into longer DNA chains, and that the in vivo synthesis of yeast DNA fragments is dependent essentially on DNApolalpha and DNApoldelta, but not necessarily on DNApolepsilon. Results presented here support the views that DNApolepsilon is dispensable for yeast DNA replication or that DNA polalpha and DNApoldelta are epistatic to DNApolepsilon in yeast.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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