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Reference: Behrens MM and Mazon MJ (1988) Yeast cAMP-dependent protein kinase can be associated to the plasma membrane. Biochem Biophys Res Commun 151(1):561-7

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Abstract

Using an anti-yeast regulatory subunit antibody and the synthetic peptide Kemptide as specific substrate we show in this work that purified preparations of yeast plasma membrane have an associated form of the regulatory subunit and cAMP-dependent protein kinase activity. Treatment of the plasma membrane "in vitro" with 1 microM cAMP releases cAMP-independent protein kinase activity while regulatory subunit remains on the membrane as revealed by immunoblotting. Incubation of the plasma membrane with [gamma-32P]ATP results in the phosphorylation of the regulatory subunit.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Behrens MM, Mazon MJ
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