The transcription of STA1 encoding an extracellular glucoamylase requires three positive regulatory genes, GAM1-3. To elucidate the role of GAM3 protein and its interaction with GAM1/SNF2 protein in transcription, we have cloned the GAM3 gene by complementation of the defect in glucoamylase production caused by a gam3-1 mutation. Genetic analysis showed that the GAM3 gene is the same as ADR6 gene which is required for transcription of the ADH2 encoding a glucose-repressible alcohol dehydrogenase II, and that gam3::URA3 strains were incapable of growing on acetate, and gam3::URA3 homozygotes failed to arrest as unbudded cells or undergo meiotic nuclear division in sporulation medium. RNA blot analysis indicated that the STA1 RNA was absent in either a gam3-1 or gam3::URA3 strain, and that accumulation of GAM1 RNA was not regulated by GAM3, likewise, GAM3 expression was not regulated by GAM1. We can conclude that GAM3/ADR6 protein is required for transcription of both STA1 and ADH2 and also for normal growth and the initiation of meiosis.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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