Chemical modification was used to study the conformational changes occurring in yeast tRNAPhe after the Y-base excision. The chemical probe was the adenine- and cytosine-specific reagent chloroacetaldehyde. Comparison of the modification patterns in tRNAPhe and tRNAPhe-Y shows that seven bases, adenines 35, 36 and 38 in the anticodon loop and adenines 73, 76 and cytosines 74, 75 in the 3'-terminus were modified in both tRNAs with a quantitative difference in the modification level of the anticodon loop bases. The most interesting, however, is the qualitative difference consisting in modification of cytosine-60 in the T psi C loop of tRNAPhe-Y. Some aspects of the mechanism of this long-distance conformational transition are briefly discussed.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|