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Reference: Lorch Y and Kornberg RD (1994) Isolation of the yeast histone octamer. Proc Natl Acad Sci U S A 91(23):11032-4

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Abstract

Procedures for the extraction and purification of the yeast histone octamer are described. Either mechanical disruption, yielding chromatin fragments, or spheroplast formation with subsequent nuclear isolation was employed. A hexahistidine tag was inserted in the N-terminal region of histone H2B, permitting resolution of the histone octamer from high-salt extracts of nuclei or chromatin to near homogeneity. The histone octamer purified in this way was fully active in reconstitution of nucleosomes.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Lorch Y, Kornberg RD
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