A 69 bp yeast genomic DNA fragment encoding yeast thioltransferase was amplified by PCR technique. A yeast genomic DNA library was screened by a specific probe obtained from the PCR product. An 718 bp DNA fragment was found to encode yeast thioltransferase and its flanking sequence. The deduced amino acid sequence of the gene, designated TTR, agrees with that derived from conventional amino acid sequence analysis except two extra amino acids on the C-terminus. In contrast to yeast thioredoxin, Southern blot analysis of total yeast genomic DNA indicated that there was only one copy of gene encoding yeast thiotransferase. A putative TATA box was found at 109 bp from the starting codon. However, no polyadenylation signal sequence was identified on the DNA sequence downstream the 3' end of the gene.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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