Nuclear respiratory deficient mutants of Saccharomyces cerevisiae have been screened for lesions in genes affecting ubiquinol-cytochrome c reductase activity. In the present study we describe a new gene, CBP4, whose encoded product is absolutely essential for the activity and expression of the respiratory enzyme. We have cloned and sequenced CBP4, which encodes a 20-kDa protein having no obvious homology to any known protein. cbp4 mutants are unable to respire due to specific loss of ubiquinol-cytochrome c reductase activity. cbp4 mutants demonstrate a pleiotropic reduction in the steady state levels of four subunits of ubiquinol-cytochrome c reductase, namely core 4, core 5, the Rieske protein and cytochrome b. Cytochrome b is not spectrally visible in the mutants, although transcription and translation of the apoprotein is normal. Antiserum prepared against a trpE/CBP4 gene fusion react with an 18.4-kDa mitochondrial protein in wild type yeast, but the protein is not found in a mutant containing a deletion of 86% of the CBP4 coding region. CBP4 protein is tightly associated with the mitochondrial membrane as evidenced by the association of the protein with the membrane fraction following carbonate extraction. The phenotype of cbp4 mutants is similar to that of cbp3 mutants. CBP3 encodes a second protein essential for ubiquinol-cytochrome c reductase activity. We found that loss of CBP3 protein does not affect the membrane stability or steady state concentration of CBP4 protein. A double mutant containing a deletion in both CBP3 and CBP4 was constructed. Our study shows that the phenotype of the double mutant is identical to the phenotypes of the individual single mutants.

• PMID: 8063753
• PubMed

Reference Type

Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, Non-P.H.S.

Authors

Crivellone MD

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