The human homolog of the S. cerevisiaelN. crassa mitochondrial protein import receptor, Mas20p/MOM19, has been identified and characterized. Sequence similarities between these three proteins is most pronounced within the NH2-terminal third of the molecules. However, the mammalian protein exhibits only weak homology to the tetratricopeptide repeat B domain that is found in Mas20p/MOM19. huMas20p is targeted and inserted into the outer membrane of isolated rat heart mitochondria, in the Nin-Ccyto orientation. Antibodies directed against the soluble portion of huMas20p inhibited in vitro mitochondrial import of a diverse set of precursor proteins (including inner membrane uncoupling protein), but failed to block import of a fusion protein bearing the signal-anchor sequence of Mas20p itself. Finally, expression of huMAS20 complemented the respiratory defect of delta mas20 yeast cells. Together, these results demonstrate that huMAS20p is a component of the mammalian import apparatus.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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