Reference: Broach JR, et al. (1979) Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8(1):121-33

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Abstract

We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2 mu circule. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Broach JR, Strathern JN, Hicks JB
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