Reference: Flanagan PM, et al. (1990) Resolution of factors required for the initiation of transcription by yeast RNA polymerase II. J Biol Chem 265(19):11105-7

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Abstract


Fractionation of a yeast nuclear extract reveals at least four factors required in addition to RNA polymerase II for accurate initiation of transcription. One of these factors can be replaced by HeLa transcription factor IID or by its yeast counterpart expressed in Escherichia coli. Each of the remaining three factors can be replaced by a fraction from yeast whole cell extract, facilitating further purification of the factors.

Reference Type
Journal Article
Authors
Flanagan PM, Kelleher RJ, Feaver WJ, Lue NF, LaPointe JW, Kornberg RD
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